| Mar 28, 2025 |
Artificial cells using riboswitch-gene fusion in liposomes produce fluorescent proteins when detecting specific targets. Multiple cell types can simultaneously detect different targets.RetryClaude can make mistakes. Please double-check responses.
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(Nanowerk News) Researchers from Ehime University created novel artificial cells by encapsulating a riboswitch-gene fusion with wheat germ extract in liposomes. These non-living cells promoted the expression of a glowing protein encoded in the gene in response to an external target at room temperature. They (precisely, a riboswitch in them) can be rationally designed for a user-defined target, allowing a cocktail of artificial cells (each group has a specific riboswitch-gene fusion) to simultaneously detect multiple targets with different glowing proteins.
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The findings are published in ACS Synthetic Biology(“Simultaneous Detection of Multiple Analytes at Ambient Temperature Using Eukaryotic Artificial Cells with Modular and Robust Synthetic Riboswitches”).
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| Simultaneous detection of multiple target molecules by a cocktail of artificial cells. When an external target molecule enters an artificial cell by membrane permeation, if a riboswitch-gene fusion specific to the target is present inside, a glowing protein is expressed from the gene via binding between the target and the riboswitch. It is possible to change the color of the glowing protein for each target, allowing multiple targets to be detected simultaneously in different colors. See the above paper (open access) for the experimental results. (Image: Atsushi Ogawa, Ehime University)
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Cell-free systems, which can express an easily detectable protein with a DNA or mRNA template without constraints of living cells, are attractive as foundations for biosensors. Moreover, by encapsulating them in lipid bilayer membranes (such as liposomes) like natural cells, these systems can avoid the adverse effects of surrounding expression inhibitors.
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In order for a cell-free system-based sensor to recognize a target molecule and subsequently express a reporter protein (e.g. glowing protein), target-to-protein signal transduction must be introduced. A promising candidate for this purpose is a riboswitch, a molecule-responsive gene-regulatory sequence. When it is fused to a reporter protein gene, it can regulate (repress or promote) the protein expression in response to a specific molecule.
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Although the diversity of natural riboswitches (or that of their target molecules) is limited, a riboswitch responsive to a user-defined molecule can be artificially created. Some natural or synthetic upregulating riboswitches (each fused to a reporter gene) have been used with cell-free systems to create artificial cell-based sensors for detecting membrane-permeable targets.
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However, all such sensors reported to date are based on prokaryotic cell-free systems and therefore do not function well at room temperature. There are also problems with riboswitch design that make it difficult to expand the variety of target molecules.
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The researchers thus utilized a eukaryotic cell-free system (wheat germ extract), which functions over a wide range of room temperature, and a highly modular synthetic riboswitch (upregulating one) that functions efficiently there. For the latter, they can change the target specificity simply by replacing the target recognition domain in the riboswitch based on their unique rational design method.
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In fact, they created three types of synthetic riboswitches (each for one of three target drugs) and fused each to the corresponding gene encoding a reporter protein (green, red, or blue glowing protein). They then encapsulated one of the fusions with wheat germ extract in natural cell-sized liposomes to create artificial cells, each glowing with an intensity dependent on the concentration of its target outside and a color specific to the target. In addition, due to their high orthogonality, a cocktail of these artificial cells allowed for simultaneous detection of the three targets at room temperature.
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